FIGURE 2.
β-Catenin (A), pGSK-3β (ΒB), and APC (C) expression in orthotopic MDA-MB-231 mammary tumors of female nude mice fed control or 5% BB diet for 7 wk. (A) Immunostaining staining intensity was graded as 1 (weak), 2 (moderate), or 3 (strong). (B,C) Three separate Western blots were quantified using Quantity One Software. Data are mean ± SEM, n = 15 (A) or 3 (means of triplicates) (B,C). Different from control, *P ≤ 0.05. APC, adenomatous polyposis coli; BB, blueberry; p-GSK, phospho-glycogen synthase kinase.