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. 2011 Sep 16;6(9):e23309. doi: 10.1371/journal.pone.0023309

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Coddens et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Anomeric regions of the 600 MHz proton NMR of the F4-binding glycosphingolipid (fraction C:tetra-I) from chicken erythrocytes (30°C). The sample was dissolved in dimethyl sulfoxide-D₂O (98∶2, by volume) after deuterium exchange. Below each section the corresponding DQF-COSY spectrum showing mainly the H1/H2 connectivities are displayed. Connectivities stemming from the same structure are color-coded by superimposed ellipses. Thus, the Forssman pentaglycosylceramide (Fo; GalNAcα3GalNAcß3Galα4Galß4Glcß1Cer) and globoside (GbO₄; GalNAcß3Galα4Galß4Glcß1Cer) are black, whereas the two novel four-sugar compounds B and C are colored blue and red, respectively. Furthermore, only connectivities other than H1/H2 ones are denoted as such.