Chemical detection by anisaldehyde (A), and autoradiogram obtained by binding of 125I-labeled F4ab fimbriae (B). The lanes were: Lane 1, globotriaosylceramide (Galα4Galß4Glcß1Cer) of human erythrocytes with d18:1-16:0 and d18:1-24:0 ceramide, 4 µg; Lane 2, globotriaosylceramide of rat intestine with t18:0-20:0-24:0 and t18:0-h22:0-24:0 ceramide, 2 µg; Lane 3, globotriaosylceramide of human meconium with t18:0-22:0-24:0 ceramide, 2 µg; Lane 4, globotriaosylceramide of human kidney with d18:1-h16:0 and t18:0-h22:0-h24:0 ceramide, 2 µg; Lane 5, isoglobotriaosylceramide (Galα3Galß4Glcß1Cer) of cat intestine with t18:0-h22:0-h24:0 ceramide, 2 µg; Lane 6, lactosylceramide (Galß4Glcß1Cer) of dog intestine with t18:0-h16:0-h24:0 ceramide, 4 µg; Lane 7, lactosylceramide of human neutrophils with d18:1-16:0 and d18:1-24:1 ceramide, 4 µg.