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. 2011 Sep 16;6(9):e23309. doi: 10.1371/journal.pone.0023309

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Coddens et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chemical detection by anisaldehyde (A), and autoradiograms obtained by binding of ¹²⁵I-labeled F4ab fimbriae (B), F4ac fimbriae (C), and F4ad fimbriae (D). The lanes were: Lane 1, sulfatide (SO₃-3Galß1Cer) of human intestine with t18:0-h24:0 ceramide, 4 µg, and globotetraosylceramide (GalNAcß3Galα4Galß4Glcß1Cer) of human erythrocytes with d18:1-16:0-24:0 ceramide, 4 µg; Lane 2, glucosylceramide (Glcß1Cer) of porcine kidney with d18:1/t18:0-16:0-24:0 ceramide, 4 µg, and neolactotetraosylceramide (Galß4GlcNAcß3Galß4Glcß1Cer) of human neutrophils with d18:1-16:0 and 24:1 ceramide, 4 µg; Lane 3, galactosylceramide (Galß1Cer) of bovine brain from Sigma-Aldrich with d18:1-h18:0-h24:0 ceramide, 4 µg, and gangliotetraosylceramide (Galß3GalNAcß4Galß4Glcß1Cer) of mouse intestine with t18:0-h16:0 and h24:0 ceramide, 4 µg; Lane 4, lactosylceramide (Galß4Glcß1Cer) of dog intestine with t18:0-h16:0-h24:0 ceramide, 4 µg, and B5 pentaglycosylceramide (Galα3Galß4GlcNAcß3Galß4Glcß1Cer) of rabbit erythrocytes with d18:1-16:0 and 24:0 ceramide, 4 µg; Lane 5, galabiaosylceramide (Galα4Galß1Cer) (synthetic) with d18:1-16:0-18:0 ceramide, 4 µg, and P1 pentaglycosylceramide (Galα4Galß4GlcNAcß3Galß4Glcß1Cer) of human erythrocytes with d18:1-16:0 and 24:0 ceramide, 4 µg; Lane 6, globotriaosylceramide (Galα4Galß4Glcß1Cer) of rat intestine with t18:0-h22:0-h24:0 ceramide, 4 µg; Lane 7, gangliotriaosylceramide (GalNAcß4Galß4Glcß1Cer) of guinea pig erythrocytes with d18:1-16:0 and 24:0 ceramide, 4 µg. The glycosphingolipids visualized with anisaldehyde in (A) are marked with Roman numbers, and the corresponding glycosphingolipid structures are given below the chromatograms.