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. 2011 Sep 16;6(9):e23309. doi: 10.1371/journal.pone.0023309

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Coddens et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Chemical detection by anisaldehyde (A), and autoradiograms obtained by binding of ¹²⁵I-labeled F4ab fimbriae (B), F4ac fimbriae (C), and F4ad fimbriae (D). The lanes were: Lane 1, glucosylceramide (Glcß1Cer) of porcine kidney with d18:1/t18:0-16:0-24:0 ceramide, 4 µg, and globotetraosylceramide (GalNAcß3Galα4Galß4Glcß1Cer) of human erythrocytes with d18:1-16:0-24:0 ceramide, 4 µg; Lane 2, galactosylceramide (Galß1Cer) of bovine brain from Sigma-Aldrich with d18:1-h18:0-h24:0 ceramide, 4 µg, and Forssman pentaglycosylceramide (GalNAcα3GalNAcß3Galα4Galß4Glcß1Cer) of dog intestine with d18:1-16:0 and 24:0 ceramide, 4 µg; Lane 3, lactosylceramide (Galß4Glcß1Cer) with t18:0-h16:0-h24:0 ceramide of dog intestine, 4 µg, and neolactotetraosylceramide (Galß4GlcNAcß3Galß4Glcß1Cer) of human neutrophils d18:1-16:0 and 24:1 ceramide, 4 µg; Lane 4, lactosylceramide (Galß4Glcß1Cer) with d18:1-16:0-24:1 ceramide of human neutrophils, 4 µg, and B5 pentaglycosylceramide (Galα3Galß4GlcNAcß3Galß4Glcß1Cer) of rabbit erythrocytes with d18:1-16:0 and 24:0 ceramide, 4 µg; Lane 5, isoglobotriaosylceramide (Galα3Galß4Glcß1Cer) of cat intestine with t18:0-h22:0 and h24:0 ceramide, 4 µg; Lane 6, gangliotriaosylceramide (GalNAcß4Galß4Glcß1Cer) of guinea pig erythrocytes with d18:1-16:0 and 24:0 ceramide, 4 µg; Lane 7, gangliotetraosylceramide (Galß3GalNAcß4Galß4Glcß1Cer) of mouse intestine with t18:0-h16:0 and h24:0 ceramide, 4 µg. The glycosphingolipids visualized with anisaldehyde in (A) are marked with Roman numbers, and the corresponding glycosphingolipid structures are given below the chromatograms.