Table 1. Summary of glycosphingolipid structures identified by NMR in the 4–6 sugar region of fractions C:tetra-I, C:tetra-II, and C:hexa in chicken erythrocytes (the identity of the anomeric and other ring proton resonances are given in the NMR spectra shown in Figs. 4 and 5).
Structure | Trivial name | VI | V | IV | III | II* | I* | |
A | Globoside | GalNAcß3 | Galα4 | Galß4 | Glcß1 | Cer | ||
H1 | 4.509 | 4.796 | 4.244 | 4.165 | ||||
H2 | 3.75 | 3.76 | 3.43 | 3.03 | ||||
H3 | 3.48 | |||||||
B | GalNAcα3 | GalNAcß3 | Galß4 | Glcß1 | Cer | |||
H1 | 4.699 | 4.594 | — | — | ||||
H2 | 4.10 | 3.95 | ||||||
H3 | 3.55 | 3.44 | ||||||
C | GalNAcα3 | GalNAcß3 | Galα4 | Galß1 | Cer | |||
H1 | ∼4.70 | 4.524 | 4.827 | 4.077 | ||||
H2 | 4.03 | 3.75 | 3.29 | |||||
H3 | 3.48 | |||||||
D | Forssman pentaglycosylceramide | GalNAcα3 | GalNAcß3 | Galα4 | Galß4 | Glcß1 | Cer | |
H1 | 4.692 | 4.511 | 4.781 | 4.254 | 4.195 | |||
H2 | 4.10 | 4.03 | 3.76 | 3.29 | 3.02 | |||
H3 | 3.55 | 3.48 | ||||||
E | Extended x2 | GalNAcα3 | GalNAcß3 | Galß4 | GlcNAcß3 | Galß4 | Glcß1 | Cer |
H1 | 4.70 | 4.596 | ∼4.26 | 4.65 | ∼4.26 | — |
*Chemical shift values are in some cases not given due to low intensities and severe resonance overlap.