Table 3. Loci sequenced and P. andina isolates cloned.
| Locus | Lengtha | Pa isolates cloned | Primers | Ta b |
| ypt1 (IR) | 227 | EC 3399, EC 3561, EC 3563, EC 3818, EC 3821, EC 3189 | IRF – TTGCAGCACAACCCAAGACG; IRR – TGCACGTACTATTCGGGGTTC | 61C |
| ypt1 (RAS) | 544 | EC 3163, EC 3399, EC 3563, EC 3821 | RASF – CGTGTCTGCTTCTCCGTTTCG; RASR – CCAGGCTTTCGGCAAATTCC | 61C |
| ypt1 (IRRAS) | 987 | EC 3163, EC 3510, EC 3563, EC 3655, EC 3818, POX 102 | IRF – TTGCAGCACAACCCAAGACG; RASR – CCAGGCTTTCGGCAAATTCC | 61C |
| trp1 | 814 | EC 3818, POX 102 | F3 – GGGTAACATCCTGGAGGAGA; R3 - TCGTACTTGACCACGTCTGC | 63C |
| beta-tubulin | 1592 | EC 3836, POX 102 | F1 – GTCCGAATTCTCCTCAGAGC; F2 – CGCTATCGGTACACCAGCTT; F3b – ACCATAACGAAGGGAAAGG; R1 – GATGCCAAGCCACTAACCTC; R2 – CCTCATTGTCCAGGCACAT | 58C |
| PITG11126 | 788 | EC 3163, EC 3510, POX 102 | F1 – GGGGACTTCGCTGTTTGTTA; R1 – ATGTTCATGTACGGCTGACG | 59C |
a
Length of the multiple sequence alignment across all sequenced isolates.
b
Optimal annealing temperature of primers for PCR based on experience in the Grünwald lab.