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. 2011 Sep 16;6(9):e24821. doi: 10.1371/journal.pone.0024821

Figure 7. Expression of transposable elements and centromeric repeat transcripts in Dicer1 knockout testes.

Figure 7

A) Quantitative RT-PCR analysis of 28 days old Dcr(fx/fx) (WT) and Dcr(fx/fx); Ngn3Cre (KO) total testis RNA using LINE1, SINEB1 and IAP primers. No differences in transposon expression between control and knockout samples were revealed. B) Expression of major centromeric repeat sequences was induced in 28 days old knockout testes. A schematic diagram shows the organization of major repeats and locus-specific primers used in the study [46]. Only the 308 bp band was detected in our optimized conditions. Three experimental triplicates (1–3) are shown for both wild type and knockout reactions. As a control, reactions were performed with mock-transcribed cDNAs (-RT).