Table 1. Expression of selected genes of interest from the male MAG/TVD transcriptome in response to feeding/courtship1 as determined by quantitative real-time PCR.
| Category | Gene | Contig | Fold2 | P-value | Significance |
| Reproduction | Astacin metalloprotease | 03261 | 7.06:1 | 1.91 | P = 0.20 |
| “ | Serine/threonine kinase | 11582 | 1.61:1 | 0.49 | P = 0.48 |
| “ | Zinc metalloprotease | 00843 | 5.01:1 | 6.23 | P = 0.016 |
| Proteases | Cathepsin B | 00689 | 1.03:1 | 0.14 | P = 0.90 |
| “ | Cysteine protease3 | 05033 | 0.21:1 | 3.08 | P = 0.05 |
| “ | Serine protease | 12380 | 22.0:1 | 12.39 | P = 0.003 |
| “ | Trypsin | 03705 | 13.5:1 | 5.45 | P = 0.003 |
| Protease inhibitor | Serine protease inhibitor | 11029 | 0.10:1 | 7.31 | P = 0.003 |
| Oxidase stress | Thioredoxin | 09407 | 1.13:1 | 0.49 | P = 0.63 |
| Structure/adhesion | Calreticulin | 10500 | 0.39:1 | 2.29E-7 | P = 0.001 |
| “ | Keratin | 01860 | 0.89:1 | 5.14E-12 | P = 0.001 |
| “ | Laminin | 12680 | 0.45:1 | 0.0005 | P = 0.001 |
| “ | Tetraspanin | 10467 | 2.93:1 | 0.002 | P = 0.001 |
| Lipases | Phospholipase C | 06555 | 1.20:1 | 0.64 | P = 0.58 |
| Neuropep. Receptor4 | GPCR | 08424 | 2.97:1 | 3.54 | P = 0.002 |
1
Transcriptome of extract made from fed Dermacentor variabilis male accessory glands, testis and vas deferens. Males were exposed to females (defined as courtship) but were not allowed to copulate. Expression compares fed versus unfed males for the MAG/TVD.
2
Change in fed male versus unfed male MAG/TVD.
3
Equals Cathepsin L.
4
Neuropeptide Receptor.
[The individual assays for these qPCR results can be supplied by authors upon request].