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. 2011 Sep 16;6(9):e24835. doi: 10.1371/journal.pone.0024835

Figure 1. Schematic representation of the reporter, silencer, and boundary minichromosomal constructs.

Figure 1

A: Physical map of the Reporter construct containing URA3 reporter gene, TRP1-ARS1 for selection and propagation in S. cerevisiae, and AmpR for modifications in E. coli. B: Physical map of the Silencer construct containing the HML - E and I silencer elements with flanking sequences on either side of the URA3 reporter gene. C: Physical map of one of the Boundary constructs. This example contains the STAR boundary element. Other boundary constructs may contain either TEF2-UASrpg or STAR element or both positioned either downstream or upstream of the silencing elements in different orientations (see schemes in Figures 2– 6).