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. 2011 Sep 16;6(9):e25046. doi: 10.1371/journal.pone.0025046

Figure 3. Prevention of cell death by HO-1 and Bcl-XL expression.

Figure 3

(A) TG cells were treated for 48 h with either siRNA targeting HO-1, Bcl-XL, or control siRNA (QIAGEN AllStars Negative Control). Bcl-XL overexpression was achieved by transfecting the cells with pcDNA4/TO-Bcl-XL. HO-1 and Bcl-XL expression was monitored by immunoblotting. β-actin was used as an internal control. A representative immunoblot of three independent experiments is shown. (B) TG cells were infected with L. monocytogenes. The infected cells were cultured with media containing 50 µg/ml gentamicin for 2, 6, and 12 h. The cells were then washed with PBS and lysed with cold distilled water. CFU was determined by serial dilution on BHI agar plates. All values represent the average and the standard deviation of three identical experiments. (C) Cell death was determined using the JC-1 Mitochondrial Membrane Potential Assay Kit. One hundred TG cells per coverslip were examined to determine the total number of live or dead cells. All values represent the average and the standard deviation of three identical experiments. Statistically significant differences compared with the control are indicated by asterisks (*, P<0.05).