Figure 4. Prevention of infectious abortion by HO-1 and Bcl-XL expression.
(A) Pregnant mice were infected with 105 cells of L. monocytogenes in 0.1 ml of saline at day 13.5 of pregnancy with or without Co-PP treatment (5 mg/kg). At day 16.5, the placentas, fetuses, and livers were removed. HO-1 and Bcl-XL expression in the placenta was analyzed by immunoblotting. A representative immunoblot of three independent experiments is shown. (B) Survival rates were determined by the presence or absence of a heartbeat in the fetuses. (C) Livers were homogenized in saline and diluted with PBS. CFU was determined by plating the diluted samples on BHI agar plate.