Table 1. Oligonucleotide primers used for amplification of cDNAs1.
rat cDNA | Sequence forward 5′ to 3′ | Sequence reverse 5′ to 3′ |
GAPDH | TCAAGGGCATCCTGGGCTAC | CGTCAAGGTGGAGGAGTGG |
TG1 | ACCAGCAGTGGCATCTTC | AATGAAAGGTGTGTCATACTTC |
TG2 | AGAGGAGCGGCAGGAGTATG | AGGATCCCATCTTCAAACTGC |
TG3 | GAAGTCAAGGTGTGTTCC | AGATGAAGATCATGTCGAA |
TG6 | TCAATCCCTGGTGCCCAGAG | ACGCCTCGGAAGATGATGCC |
FXIIIa | TCACTGCACGCATCAACGAGAC | TGCCTCGGACCTTGATGGTGAC |
Primers were designed using the Primer Express program (Applied Biosystems) and purchased from Eurogentec (Seraing, Belgium).