Table 1. Turnover rates (min−1) of the published LinA variants towards α-, γ- and δ-HCH, along with the amino acid differences amongst thema.
Residue variations | Turnover number (min−1) | ||||||||||||||||||||||||||||
20 | 23 | 35 | 68 | 71 | 96 | 110 | 111 | 113 | 115 | 126 | 129 | 131 | 133 | 145 | 149 | 150 | 151 | 152 | 153 | 154 | 155 | 156 | α | γ | α/γ | δ | |||
Group 1 | A | LinA2B90A | K | A | I | F | C | L | A | A | F | D | F | R | A | T | A | I | H | F | A | P | S | G | A | 3815±3 | 733±13 | 5.2 | 174±4 |
LinAγ1-7 | . | . | . | . | . | . | T | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 3001±30 | 11794±526 | 0.2 | 284±32 | ||
LinAbITRC-5 | . | . | . | . | . | . | T | C | . | . | . | . | . | . | . | . | . | . | T | . | . | . | . | 2306±29 | 2778±180 | 0.8 | 246±3 | ||
LinANM05 | . | . | . | . | . | . | T | C | . | . | . | . | . | . | . | . | . | L | . | . | T | . | . | 11267±27 | 889±107 | 12.6 | 10±1 | ||
b | LinADS3-1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | A | L | L | Q | K | . | . | . | 7884±14 | 5376±359 | 1.4 | 214±35 | |
Group 2 | a | LinAaITRC-5 | . | G | V | Y | T | C | . | . | Y | N | . | L | G | M | V | A | . | . | . | . | . | – | – | 965±0 | 65±15 | 14.8 | 49±1 |
b | LinA1B90A | Q | G | . | Y | T | C | . | . | Y | N | L | L | G | M | . | A | L | L | Q | K | . | – | – | 418±4 | 89±7 | 4.6 | 10±1 |
LinA2B90A derives from Sphingobium indicum B90A and is identical to LinA from Sphinogobium sps. strains UT26, Sp+, DS2, γ16-1, BHC-A and Rhodanobacter lindaniclasticus; LinAγ1-7 is from Sphingomonas sp. γ1-7; LinAbITRC-5 is from Pseudomonas aeruginosa ITRC-5; LinANM05 comes from Sphingomonas sp. NM05; LinADS3-1 is from Sphingomonas sp. DS3-1 and is identical to LinA of Sphingobium sp. α1-2; LinAaITRC-5 is from Pseudomonas aeruginosa ITRC-5; LinA1B90A is from Sphingobium indicum B90A and is identical to LinA of Sphingomonas sp. α4-2 [2].