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. 2011 Sep 16;6(9):e25188. doi: 10.1371/journal.pone.0025188

Figure 1. Schizosaccharomyces Ofd2 belongs to the AlkB subfamily of 2OG/Fe(II) dioxygenases.

Figure 1

(A) A multiple sequence alignment (MSA) of the 2OG/Fe(II) dioxygenase core segment of S. pombe Ofd2 (residues 114–220) and homologous sequences from three other Schizosaccharomyces species, the fungi Ustilago maydis, Penicillium chrysogenum, and Laccaria bicolor, S. pombe SPBC13G1.04c, human ALKBH1-8, and E. coli AlkB, show that Ofd2 is an AlkB family member with conserved metal and 2OG binding residues. The eight β-strands of the E. coli AlkB core domain, shown as arrows below the MSA, are conserved in Ofd2. Gaps and deleted sequence are shown as dashes and dots, respectively. NCBI sequence identifiers [43] are shown where these are available. (B) A structural model of the S. pombe Ofd2 2OG/Fe(II) dioxygenase core shows the ‘jellyroll’ structural fold with the eight β-strands (same coloring as in panel A) and the location of the conserved 2OG (cyan sticks) and Fe(II) (purple ball) complexing residues.