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. 2011 Sep 16;6(9):e25089. doi: 10.1371/journal.pone.0025089

Figure 2. Identification of the 90 KDa protein associated with NadA as hsp90 β by MS/MS.

Figure 2

The picture shows a typical Coomassie G250 profile of the proteins co-isolated with NadA/anti-NadA immune complexes. The two arrows point to the 90 KDa protein and to 35 KDa NadAΔ351–405, while H and L indicate antibody chains. The 90 KDa was excised from the gel for identification by MS/MS sequencing after trypsinization and identified as the human hsp90 β. The MS full scan (A) and data dependent MS/MS sequencing scans of the high mascot score peptide candidate 764.52 m/z (B) are shown, followed by a table of its Y and B ions (Table 1).