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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Gene Ther. 2011 May 26;19(1):101–108. doi: 10.1038/gt.2011.76

Figure 5.

Figure 5

The effect of T0TNFSR or T0Z on the expression of mRNA of spinal TNFα and IL-1β. The non-replicating herpes simplex virus (HSV) vector T0TNFSR producing p55 TNFSR, and control vector T0Z were used. Rats received a repeated morphine dose at 1 week after vectors. In the sham group, rats receive T0Z and a repeated dose of saline. At day 7, 1 hour after the last dose of morphine or saline, L4/5 spinal dorsal horns were harvested, and the expression of TNFα and IL-1β was tested using quantitative real time PCR. Repeated morphine significantly induced upregulation of spinal TNFα (A) and IL-1β (B). The expression of TNFα and IL-1β mRNA in morphine tolerance rats with T0TNFSR was significantly lower than that in morphine tolerance rats with T0Z. # p<0.05, ## p<0.01 vs sham; * p < 0.05, **p<0.01vs T0Z, n=4–6, ANOVA, post hoc comparisons using Fisher's PLSD test (StatViewJ 5.2).