Fig. 2. Regulation of 14-3-3ζ by miR-451 overexpression in MCF7 and TamR cells.
A-B) Overexpression of miR-451 resulted in the downregulation of 14-3-3ζ mRNA (quantified by RT-qPCR) and protein (by Western blot). Control (Ctrl) cells received vector only. Each experiment was performed in triplicate and mean ± SD is shown. C) Expression of five 14-3-3 family members in MCF7 cells without and with miR-451 overexpression. **, p<0.01.