Fig. 4. Increasing the expression of miR-451 reduces 14-3-3ζ and stimulates apoptosis, and the effects of miR-451 are reversed by expression of 14-3-3ζ.

A) Western blots of whole cell extracts from vector, miR-451 alone, or miR-451 and 14-3-3ζ transfected MCF7 cells treated with Tam for 0, 24, or 48h and probed with antibodies against 14-3-3ζ and caspase-7 to detect intact and cleaved CASP7 products. β-actin was used as loading control. B) Control vector, miR-451, or miR-451 and 14-3-3ζ-expressing Tam^R cells were treated with 1μM Tam or control vehicle for 72 h, then fixed and stained with propidium iodide, and cell cycle distribution was monitored by flow cytometry. C) The percent of apoptotic cells (sub-G1 peak) in control vector (Ctrl), miR-451, and miR-451 and 14-3-3ζ -expressing cells is presented. ***, P < 0.001.