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Visualization of a genetically encoded O-allyltyrosine-containing Z-domain protein in E. coli via the photoinduced tetrazole-alkene cycloaddition reaction: (a) Reaction scheme; (b) CFP (cyan fluorescent protein) channel (top panels) and DIC (Differential Interference Contrast) channel (bottom panels) images of bacteria expressing either alkene-Z (left) or wt-Z (right) proteins after treatment with 100 μM of tetrazole 15; scale bar = 10 μm.
