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. 2011 Nov;1808(11-15):2638–2645. doi: 10.1016/j.bbamem.2011.07.026

Fig. 1.

Fig. 1

Top: Flow cytometry plots of Annexin V-FITC/PI labeled (black filled) and unlabeled cells (grey line) comprising differentiated control cells of primary cell cultures (diff pcc) of synoviocytes, isolated from synovium of Osteoarthritis (OA) patients and tumor cell lines (cl) of different cancer types, as rhabdomyosarcoma, a malignant soft tissue tumor of mesenchymal origin (TE671), prostate cancer (LNCaP) and renal cell cancer (769-P). Plots indicate a strong binding of Annexin V to PS on tumor cell lines. Second panel: Flow cytometry plots of Annexin V-FITC/PI labeled (black filled) and unlabeled cells (grey line) comprising differentiated control cells of primary cultures (diff pcc) of melanocytes (FOM101) and melanoma cell lines (cl) of primary lesions (pl) (nt non-tumorigenic) (SBcl-2) and more highly malignant melanoma metastases (met) (WM9 and WM164). Annexin V binds significantly to melanoma cell lines of primary and metastatic lesions. Fluorescence microscopy pictures (overlay of bright field and fluorescence channels) of melanocytes and melanoma cell lines indicate specific binding of Annexin V-Alexa Fluor 488 (green) to PS on the outside of melanoma cells. No PI (red) is integrated assuring integrity of cells studied.