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Selection of parameters for ASE isolation of ZON and its metabolite
| No. | Tissue | Solvent or combination of solvents, % v/v | Quantity of used mixture, ml | Number of extraction cycles | Temperature, °C |
|---|---|---|---|---|---|
| 1 | Veal muscle tissue | MeOH | 42 | 2 | 110 |
| 2 | ACN/H2O 84:16 | ||||
| 3 | ACN/MeOH 84:16 | ||||
| 4 | ACN/MeOH 84:16 | 44 | 4 | ||
| 5 | Poultry muscle tissue | ACN/MeOH 84:16 | |||
| 6 | ACN/MeOH 84:16 | ||||
| 7 | ACN/MeOH 84:16 | 46 | |||
| 8 | ACN/H2O 84:16 | 46 | |||
| 9 | Poultry liver | ACN/H2O 84:16 | 52 | 150 | |
| 10 | ACN/H2O 84:16 | 64 | 200 | ||
| 11 | Chloroform | 43 | 110 | ||
| 12 | ACN | 42 | 2 | 110 |
