Table 2.

PCR Primers and Product Length

Gene	Forward primer	Reverse primer	Amplicon size (bp)
KIT⁎	5′-CGTGGAAAAGAGAAAACAGTCA-3′	5′-CACCGTGATGCCAGCTATTA-3′	70
POU5F1†	5′-GAGGATCACCCTGGGATATAC-3′	5′-CGATACTGGTTCGCTTTCTCTTT-3′	295
NANOG	5′-AATACCTCAGCCTCCAGCAGATG-3′	5′-CTGCGTCACACCATTGCTATTCT-3′	149
SOX2	5′-GTTACCTCTTCCTCCCACTCCA-3′	5′-CACCCCTCCCATTTCCCTCGTT-3′	260
KLF4	5′-GGGAGAAGACACTGCGTCA-3′	5′-GGAAGCACTGGGGGAAGT-3′	88
GAPDH	5′-CGACCACTTTGTCAAGCTCA-3′	5′-AGGGGTCTACATGGCAACTG-3′	228
HPRT	5′-CGTGATTAGTGATGATGAACCAG-3′	5′-CGAGCAAGACGTTCAGTCCT-3′	129

^⁎The protein product for the KIT gene is known also as c-kit.

^†The protein product for POU5F1 is known also as OCT3, OCT4, or OCT3/4.