Figure 3.

Serum-starved endothelial cells were re-plated onto functionalized silicon surfaces for 30 min (white bars) or 3 h (black bars), fixed, stained and imaged as described for Fig. 2. The fluorescence images were analyzed to determine (A) the average number of adherent cells per area (μm²) after serum-starved cells were adhered for 30 min (white bars) and 3 h (black bars) in serum-containing media to the indicated functionalized silicon surfaces, (B) cell spreading, (C) the number of vinculin-containing FAs per area (μm²), and (D) length of vinculin-containing FAs after cells adhered for 3 h on functionalized surfaces. Error bars represent standard deviations (SDs) of (A) >100 cells and (B–D) >15 cells. ^∗p < 0.05 between the indicated surface and 100% RGD; ^∗∗p < 0.05 relative to 1:10³ RGD/EO₆ surfaces.