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. 2011 Aug 17;101(4):764–773. doi: 10.1016/j.bpj.2011.06.064

Figure 5.

Figure 5

Integrin activation. Integrin activation was determined with GST-FN9-11, which specifically binds to activated α5β1. (A) Endothelial cells that adhered to functionalized silicon surfaces for 3 h were incubated with GST-FN9-11 for 30 min in the absence or presence of 1 mM Mn2+, which activates integrins (total α5β1). Cell lysates were probed for GST. (B) Quantification of integrin activation was expressed as the ratio of activated/total α5β1, and the ratio was normalized to cells on 100% RGD surfaces. Data and error bars represent the mean ± SD of four independent experiments; ∗p < 0.05 between 100% RGD and 1:103 RGD/EO6 surfaces, with none of the other surfaces being significantly different from the 100% RGD surface.