Figure 6.

VEGF-induced signaling. (A) Endothelial cells that adhered to 1:10³ RGD/EO₆ and 1:10⁶ RGD/EO₆ surfaces for 3 h were stimulated with 25 ng/ml VEGF for 0–20 min, lysed, and analyzed by immunoblotting for the phosphorylation of FAK, PLCγ, ERK1/2, eNOS, Akt, and Src on the same blot. Probing for nonphosphorylated proteins (total), as shown for FAK, acted as a loading control. (B and C) Adherent cells on various surfaces were stimulated with VEGF stimulation for 10 min. Immunoblots were quantified by expressing the ratio of phosphorylated/total protein and normalizing the ratio to cell lysates from 100% RGD surfaces of the same blot. Data and error bars represent the mean ± SD of four independent experiments; ^∗p < 0.05 relative to 100% RGD surfaces.