Figure 8.

Migration of endothelial cells. A monolayer of endothelial cells cultured on silicon surfaces was scratched to introduce a wound with an average width of 310 ± 25 μm. After a further 3 h in the absence (A) or presence (B) of 25 ng/ml VEGF, individual cells started to migrate into the wound. Zoomed images show the elongated shape of migrating cells and the extension of filopodia (C). The distance between the cell fronts was measured for >50 different sites. The data show the mean ± SD of wound closure in the absence (white bars) and presence (black bars) of VEGF; ^∗p < 0.05 relative to 100% RGD surfaces.