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. 2011 Aug 17;101(4):L27–L29. doi: 10.1016/j.bpj.2011.04.057

Figure 1.

Figure 1

(A) Cell expressing Kapβ1-GFP (scale bar: 2 μm). (B) The PSF is scanned along a 64-point orbit of 180 nm in radius (R) around the pore (the dimensions of the pore in the drawing are in scale with the orbit radius). The first half of the orbit (points 0–32) passes over the cytoplasmic side of the pore, and the second half (points 32–63) passes over the nucleoplasmic side. (C) Modulation and xy trajectories over time (one cycle = 16 orbits). (D) Fluorescence intensity along the orbit versus time. (E and F) Total ACF carpet and zoom on the region containing humps (dashed circles). (G) Average ACF plot (black) and ACF of column 23 (red).