Figure 5.
Alcohol bilayer-perturbing effects in thinner (DC20:1PC) bilayers. Fluorophore-loaded LUVs were made using DC20:1PC, and 80 nM of a sequence-shortened gA analog (gA−(13)) were added to the gA-containing vesicles. The effects of ethanol, 1-octanol, 1-nonanol, and 1-decanol were determined at twice their doubling rate concentration, D, or 294, 0.82, 0.38, and 1.26 mM respectively. (A) Average traces without gA for control, 1-octanol, and 1-decanol (top three traces), and with added gA−(13) for control, 1-decanol, and 1-octanol (bottom three traces). (B) Average and SD of the relative change in quenching rates as a function of added alcohols.