Figure 3.

There is no fixed correlation between stem length and activity. (A - D) Four extra hairpin sets of 19, 21, 23, 25, 27 and 29 bp stems were designed to target Vpu, initiating from nucleotide positions 10, 51, 127 and 158 (relative to the start of the Vpu gene). The Vpu 158 set was expanded to include 17 and 18 bp stems. (E) Additional Tat hairpin pairs were designed to compare the activity of the most commonly employed short and long stem lengths (21 cf. 29 bp), and included the Tat56 21 and 29 bp hairpins. All values are averages from at least 3 independently repeated experiments with 95% Confidence Intervals (CI) shown.