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A 23 bp hairpin can be improved by an increase in stem length. (A) The last 6 bp of a 29 bp hairpin were replaced with extensions of varying GC contents. (B) An additional set of hairpins of 23 - 29, 33, 37 and 41 bp stems in which only the first 23 bp was matched to the target, with the remaining sequence inverted were created and tested. All values are averages from at least 3 independently repeated experiments with 95% Confidence Intervals (CI) shown.
