Table 2.
Free amino acid uptake and release during HEK293SF cell growth after infection.
| Gln | Asp | Glu | Ser | Asn | Gly | His | Thr | Arg | Ala | Pro | Cys | Val | Met | Ile | Leu | Lys | Phe | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Free amino acid level before cell infection, mM | Batch | 2.21 | 1.10 | 1.62 | 5.84 | 7.95 | 0.66 | 0.98 | 2.87 | 1.78 | 1.01 | 4.36 | 0.43 | 2.50 | 0.80 | 2.31 | 3.57 | 2.66 | 1.14 |
| Perfusion | 1.77 | 0.87 | 1.48 | 2.80 | 7.06 | 0.73 | 0.87 | 2.17 | 1.32 | 1.28 | 3.70 | 0.02 | 2.04 | 0.67 | 1.91 | 3.11 | 2.17 | 0.96 | |
| Specific uptake or release of free amino acids, μmol.10-6 cell | Batch | 0.09 | 0.00 | -0.03 | 0.01 | -0.06 | -0.03 | 0.01 | -0.05 | 0.01 | -0.09 | 0.05 | -0.04 | 0.03 | 0.01 | 0.01 | -0.01 | -0.03 | -0.01 |
| Perfusion | 0.12 | 0.02 | -0.01 | 0.08 | 0.00 | -0.06 | 0.01 | 0.03 | 0.04 | -0.08 | 0.00 | 0.02 | 0.03 | 0.01 | 0.05 | 0.09 | 0.06 | 0.01 | |
Values for specific uptake and release were calculated between infection time and the start of cell death (24 h post-infection). Positive values represent amino acid uptake, while negative ones represent their release.