Table 3.
Influenza HA production in batch and perfusion culture at different days post-infection (dpi).
| HA titers, logHA units/mL | Total productivity, logHA units | Specific productivity, HA units/105 cell | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 dpi | 2 dpi | 3 dpi | 4 dpi | 1 dpi | 2 dpi | 3 dpi | 4 dpi | 1 dpi | 2 dpi | 3 dpi | 4 dpi | |
| BATCH | 2.5 | 3.7 | 3.4 | 6.0 | 7.2 | 6.9 | 6 | 97 | 49 | |||
| PERFUSION | 1.9 | 4.3 | 4.0 | 3.8 | 5.4 | 7.8 | 7.8 | 7.7 | 0.9 | 199 | 191 | 173 |
Total viral particles were quantified by hemaglutination assays (HA titers). Total productivity was calculated at different times of harvest post-infection (Harvest of the perfused flow, 1.5 L/day, was taken into account in the calculation for perfusion system). Specific productivities were calculated based on total productivity and the maximum cell density attained (5.76 × 106 cell/mL in batch and 10.1 × 106 cell/mL in the perfusion system).