Table 4.
Influenza infectious particle production in batch and perfusion cultures at different days post-infection (dpi).
| TCID50 titers, TCID50 units/mL | Total productivity, TCID50 units | Specific productivity, TCID50 units /103 cell | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 dpi | 2 dpi | 3 dpi | 4 dpi | 1 dpi | 2 dpi | 3 dpi | 4 dpi | 1 dpi | 2 dpi | 3 dpi | 4 dpi | |
| BATCH | 3.0 × 109 | 5.3 × 1010 | 3.0 × 108 | 1.0 × 1013 | 1.6 × 1014 | 7.9 × 1011 | 0.7 | 10.4 | 0.1 | |||
| PERFUSION | 2.0 × 109 | 2.1 × 1010 | 3.3 × 1011 | 2.0 × 109 | 7.9 × 1012 | 1.6 × 1014 | 1.0 × 1015 | 2.5 × 1014 | 0.3 | 4.9 | 36.8 | 8.5 |
Total productivity was calculated at different times of harvest post-infection (Harvest of the perfused flow, 1.5 L/day, was taken into account in the calculation for total productivity in the perfusion system). Specific productivities were calculated based on total productivity and maximum cell density attained (5.76 × 106 cells/mL in batch and 10.1 × 106 cells/mL in the perfusion system).