Figure 2.
Amplification plots and standard curves of the five duplex real-time PCRs. Typical amplification plots for each viral target and the corresponding internal report in theirs linear dynamic ranges are represented. The corresponding standard curves are obtained by linear regression analysis of the threshold cycle (Ct) value of each of the two standard-dilution replicates over the log10 of the copies of DNA targets. The slope and the correlation coefficients are mentioned for each standard curve.