Table 2.
Genes selected for the real-time qRT-PCR validation experiments
| Organ | Symbol | Definition | Accession | Assay ID |
|---|---|---|---|---|
| Liver | Cyp3a11 | Mus musculus cytochrome P450, family 3, subfamily a, polypeptide 11 | NM_007818 | Mm00731567_m1 |
| Liver | Hmgcs1 | Mus musculus 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 | NM_145942 | Mm00524111_m1 |
| Spleen | Vwf | Mus musculus Von Willebrand factor homologue | NM_011708 | Mm00550376_m1 |
| Spleen | Brca1 | Mus musculus breast cancer 1 | NM_009764 | Mm00515386_m1 |
| Heart | Pdk4 | Mus musculus pyruvate dehydrogenase kinase, isoenzyme 4 | NM_013743 | Mm00443325_m1 |
| Heart | Rad21 | Mus musculus RAD21 homologue (S. pombe) | NM_009009 | Mm00485474_m1 |
| All | Sfrs9 | Mus musculus splicing factor, arginine/serine rich 9 | NM_025573 | Mm00470546_m1 |
| All | Guk1 | Mus musculus guanylate kinase 1 | NM_008193 | Mm00433888_m1 |
| All | Hnrpab | Mus musculus heterogeneous nuclear ribonucleoprotein A/B | NM_010448 | Mm00468938_m1 |
These six target genes were selected based on their presence in significant functions, their differential scores, their detection levels, their presence as single splice transcripts in microarrays and their availability as Taqman assays designed across splice junctions. Sfrs9, Guk1 and Hnrpab were used as housekeeping genes as their expression levels were found to be quite stable across treatments in each of the organs tested.