Figure 5.

Microarray and RT-qPCR gene expression profiles of the G. hirsutum gene harboring the NAU3991 EST-SSR marker. The cotton gene with the NAU3991 EST-SSR marker is designated "putative plectin-related protein" based on sequence similarity to a putative A. thaliana orthologue. (A) The transcript abundance of the gene in Li₂ mutant and WT fibers as determined by microarray. Relative expression represents signal intensity of probesets. Error bars indicate standard deviation from 2 biological replicates. The DPA time-points that revealed a significant (≥ 2-fold; < Bonferroni-corrected p-value threshold 2.07194E-06) are indicated by an asterisk and the fold-change in transcript abundance is shown on the graph above each indicated time-point. (B) The transcript abundance of the gene in Li₂ mutant and WT fibers as determined by RT-qPCR. Error bars indicate standard deviation from 3 biological replicates. The gene is represented by the indicated TC in the Cotton Gene Index 11.0 database (http://compbio.dfci.harvard.edu/tgi/). The DPA time-points that revealed a significant (≥ 2-fold; p-value < 0.05) difference in transcript abundance are indicated by an asterisk and the fold-change in transcript abundance is shown on the graphs above each indicated time-point.