Low concentrations of agonistic neurosteroids cause current influx at α1S299C containing receptors. Tracings are examples of 3–31 samples. A: Representative tracings from the same oocyte expressing α1S299C containing receptors in the presence of 1 mM GABA, then 100 nM ALLO, then 5 nM ALLO. Each ALLO concentration passes approximately 50% of 1 mM GABA current. B: Representative trace of α1S299C containing receptors in the presence of 1 mM GABA, then 100 nM THDOC, with THDOC passing approximately 50% of 1 mM GABA current. C: In a different oocyte, representative trace of α1S299C containing receptors in the presence of 1 mM GABA, then 1 nM THDOC, with 1 nM THDOC passing more than 50% of 1 mM GABA current. For all panels, cells were from different experiments on different days with different injections into different oocytes; hence the variability in the amount of induced currents and the experiments in panel A taking longer to reach a clear maximum.