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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Nature. 2011 Aug 28;477(7364):354–358. doi: 10.1038/nature10379

Figure 2. CENP-A chromatin specifically recruits kinetochore proteins as a response to a mimic of kinetochore detachment from microtubules.

Figure 2

(a) A schematic showing the experimental procedure. (b) Quantification of immunofluorescence analysis of CENP-C, Ndc80, CENP-E, Mad2, Rod or ZW10 recruitment to chromatin arrays with (+) and without (−) nocodazole (NOC). The levels are rescaled so that CENP-A arrays with (+) nocodazole are set at 1. Error bars represent SEM, n = 3 (p < 0.05 between − and + nocodazole for CENP-E, Mad2, Rod and ZW10 binding to CENP-A chromatin arrays). (c) Western blot analysis of CENP-C, Ndc80, Rod and ZW10 recruitment to CENP-A and H3 chromatin arrays with and without (+/−) nocodazole (NOC) in CSF and cycled egg extracts. H4 levels are shown as a loading control.