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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Nature. 2011 Aug 24;477(7364):340–343. doi: 10.1038/nature10348

Figure 1. A haploid genetic screen identifies the HOPS complex and NPC1 as host factors for filovirus entry.

Figure 1

a, Genes enriched for gene-trap insertions in the rVSV-GP-EboV-selected cell population compared to unselected control cells. Circles represent genes and their size corresponds to the number of independent insertions identified in the rVSV-GP-EboV selected population. Genes are ranked on the X-axis based on chromosomal position. b, RT-PCR analysis of the expression levels of NPC1, VPS33A and VPS11 in mutant clones. c, Infectivity of VSV pseudotyped with the indicated filovirus glycoproteins. Means ± standard deviation (SD) (n=3) are shown. EboV, Ebola virus (Zaire), MarV, Marburg virus. *below detection limit. d, HAP1 clones were infected with viruses including recombinant VSV viruses carrying rabies or Borna disease virus glycoproteins (rVSV-G-RABV and rVSV-GP-BDV) and stained with crystal violet.