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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Nature. 2011 Aug 28;477(7364):295–300. doi: 10.1038/nature10398

Figure 1. Functional effects of lincRNAs.

Figure 1

(a) A schematic of lincRNA perturbation experiments. ESCs are infected with shRNAs, knockdown level is computed, the best hairpin is selected and profiled on expression arrays, and differential gene expression is computed relative to negative control hairpins. (b) Example of a lincRNA knockdown. Top: Genomic locus containing the lincRNA. Bottom: Heatmap of the 95 genes affected by knockdown of the lincRNA, expression for control hairpins (red line) and expression for lincRNA hairpins (blue line) are shown. (c) Distribution of number of affected genes upon knockdown of 147 lincRNAs (blue) and 40 well-known ESC regulatory proteins (red). Points corresponding to five specific ESC regulatory proteins are marked.