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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Oncogene. 2011 Jun 13;30(45):4567–4577. doi: 10.1038/onc.2011.164

Table 2.

Number of siRNA in screen 541
Number of siRNA targets assayed for protein
expression by RPPA in screen		 15
Number (percent) of successful knockdowns in
screen (measured by RPPA)		 10/15
(67%)
Number of genes targeted that decreased pAKT
in screen (z-score < −1.4)		 34
Number of gene targeted that increased pAKT in
screen (z-score >1.4)		 15
Number of gene targets re-validated using
different siRNA sequences		 18
Number (percent) of genes that re-confirmed
knockdown with different siRNA		 13/18
(72%)
Number (percent) of genes targeted that reconfirmed
change in pAKT with different siRNA		 10/13
(77%)
Number (percent) of genes targeted that showed
no change in pAKT with different siRNA		 2/13
(15%)
Number (percent) of genes targeted that showed
opposite change in pAKT with different siRNA		 1/13
(8%)