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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Transfusion. 2011 Jun 3;51(12):2695–2702. doi: 10.1111/j.1537-2995.2011.03197.x

Figure 1. Concurrent transfusion of fresh HOD RBCs with stored HOD RBCs decreases the proinflammatory cytokine storm seen following the transfusion of stored HOD RBCs alone.

Figure 1

Wild-type C57BL/6 recipient mice were transfused with either 1 unit (i.e. 100 microliters of packed RBCs) of fresh, leukoreduced HOD RBCs, 1 unit of 14-day stored, leukoreduced HOD RBCs, or a combination of 1 unit of fresh, leukoreduced HOD RBCs and 1 unit of 14-day stored, leukoreduced HOD RBCs. In the combined group, the units were transfused minutes apart and into different tail veins. Recipient cytokine responses were evaluated in sera collected 90 minutes following transfusion. Concurrent transfusion of fresh plus stored HOD RBCs blunted the cytokine response of IL-6 (A), KC (B), and MCP-1 (C), as compared to stored RBCs alone, in a statistically significant manner (*p<0.05 by one way ANOVA with Bonferroni post-test). No statistically significant differences in recipient serum levels of IL-10 (C), IFN-γ (E), or TNF-α (F) were seen. A compilation of data from 3 independent experiments (4–5 mice/group/experiment) is shown.