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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: J Org Chem. 2011 Jun 15;76(14):5614–5627. doi: 10.1021/jo200482d

Figure 9.

Figure 9

Result of a gel-shift assay following incubation of a 171bp, linear double-stranded DNA with different concentrations of PNA6 and PNA10 in 10 mM sodium phosphate buffer (pH 7.4) at 37 °C for 16 hr, followed by electrophoretic separation on nondenaturing-gel and SYBR-Gold staining. Note that this particular DNA fragment, which was PCR-amplified from a Psuper plasmid vector, does not have sequence complementary to PNA6 or PNA10 (both contained the same nucleobase sequence). The concentration of the 171bp DNA fragment was 0.4 μM.