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Concentration dependent 125I-VEGF binding to immobilized rFn fragments. 125I-VEGF was incubated for 2 hours on wells coated with increasing concentrations of rFn fragments. After washing, bound VEGF was eluted with 0.1 mol/L NaOH/1% SDS and radioactivity quantified in a gamma counter. Data are presented as the mean of triplicate determinations±SEM.
