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. Author manuscript; available in PMC: 2011 Sep 18.
Published in final edited form as: Circ Res. 2006 Sep 28;99(8):853–860. doi: 10.1161/01.RES.0000246849.17887.66

Figure 7.

Figure 7

rFnIII9–10/12–14 promotes Erk and VEGFR-2 phosphorylation at low VEGF concentration. A and B, HUVECs were exposed to varying concentrations of VEGF in the absence (A) or presence (B) of rFnIII9–10/12–14 (0.2 μmol/L). Erk phosphorylation was determined by Western blotting. A representative blot from 2 separate experiments is shown. C, HUVEC cultures were stimulated with a low dose of VEGF (1 ng/mL) in the absence or presence of recombinant Fn fragments (0.2 μmol/L). Cell lysates were immunoprecipitated with anti–VEGFR-2 rabbit monoclonal antibodies followed by Western blotting with anti-phosphoVEGFR-2 rabbit monoclonal antibodies. Blots were stripped and reprobed with anti–VEGFR-2 antibodies. Experiments were repeated twice with similar results.