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. 2011 Aug 31;41(4):167–175. doi: 10.5051/jpis.2011.41.4.167

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Copyright © 2011 Korean Academy of Periodontology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/).

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Morphology of the various stages and percentage of calcified areas during the formation of mineralized nodule by human periodontal ligament (PDL) cells. (A) shows the visual field at ×200 magnification on days 1(a), 7(b), 14(c), and 21(d). Developed mineralization is observed and size and number of nodules are increased compared to PDL cells on day 14. (B) Measurement of calcified areas by Alizarin Red S stain. Data are shown as the mean±SD of two patients. Percentages of the calcified areas are 5% and 33% of the culture dish on day 14 and 21, respectively.