Figure 1.
Agarose gel stained with ethidium bromide after electrophoresis. Lane 1, DNA ladder; Lanes 2-10, various plasmids digested with EcoR I and BamH I: lane 2, vector pLex5BA; lane 3, pLex5BA-fabD; lane 4, pLex5BA-fabI; lane 5, pLex5BA-murA; lane 6, pLex5BA-murG; lane 7, pLex5BA-prfB; lane 8, pLex5BA-rplJ; lane 9, pLex5BA-rpsL; lane 10, pLex5BA-trpS. Note, due to restriction sites and Shine-Delgarno sequences designed into PCR primers, the sizes of inserts shown in the gel are slightly bigger than the ORF sizes listed in Table 1.