Figure 4.

Increased resistance to triclosan via over-expression of inhibitor-specific essential gene product, FabI. The FabI clone was grown in presence of serial dilutions of triclosan either with or without 1 mM IPTG. A vector control clone containing vector alone served as a control. For each condition (in respect to inhibitor and inducer), three replicate wells were used. Late log-phase (10 h) plate read values were used to generate the dose response graph.