Figure 5.

ITGA11, ADAMTS9, ADAM12, and NID1 are direct targets of miR-29. FG293 cells were cotransfected with individual 3′ untranslated region (UTR) luciferase reporters with wild-type (wt) or mutated (mut) miR-29–binding sites, along with miRNA mimics of miR-29 or miR-365. Firefly luciferase activity from the same construct was used to normalize and to generate relative activity of Renilla luciferase, which was subject to the regulation of cloned 3′ UTR. miR-29 significantly suppressed luciferase activities of reporters harboring wild-type 3′ UTR sequences, but not for reporters with mutated miR-29–binding sites. miR-365, an unrelated miRNA, has no significant effect on the luciferase activities of reporters with either wild-type or mutant 3′ UTRs.